SCREENING BAKTERI TERMOFILIK PENGHASIL ENZIM AMILASE DARISUMBER AIR PANAS SINGGAHAN TUBAN, JAWA TIMURSCREENING THERMOPHILIC BACTERIA OF AMYLASE ENZYME FROMSINGGAHAN HOT SPRINGS TUBAN, JAWA TIMUR

YULIANA EVA NOVITASARI

Abstract


Abstrak. Penelitian ini bertujuan untuk memperoleh bakteri termofilik yang menghasilkan amilase dari
sumber air panas singgahan di Tuban berdasarkan clear zone dan menentukan spesies bakteri yang
mempunyai indeks amilolitik tertinggi. Diperoleh 103 isolat dan 51 isolat menunjukkan aktivitas amilase
dengan terbentuknya zona bening disekitar koloni pada media padat yang mengandung 1% pati. Indeks
amilolitik tertinggi ditunjukkan oleh isolat ST-32 sebesar 3,8 mm. Berdasarkan uji morfologi dan fisiologi
isolat ST-32 menunjukkan spesies Thermoactinomyces saachari.
Kata kunci: bakteri termofilik, amilase, pati, zona bening
Abstract. This research aims to obtain thermophilic bacteria produced amylase from Singgahan hot spring
in Tuban based on clear zone and determine species of bacteria that have highest amylolytic index. It was
obtained 103 isolates and 51 isolates showed amylase activity with formation clear zone around the colony
in medium containing 1 % starch. The highest amylolytic index was showed by ST-32 isolate is 3.8 mm.
Based on morphology and phisiology ST-32 isolates shown species Thermoactinomyces saachari.
Keywords: thermophilic bacteria, amylase, starch, clear zone


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